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DIPENDIX
I.DOT
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The I.DOT is the instrumen to incorporate drop detection and the capability to detect when users run out of source liquid and verifies dispensing volume.


I.DOT’s DropDetection is a patented feature that detects and counts every droplet released during a single dispensing run. It’s a simple and powerful tool enabling droplet verification. DropDetection uses a circuit board mounted under the I.DOT source tray that leverages 96 miniaturized light barriers to detect every droplet generated from each source plate posi-tion, identifying changes in light intensity to detect droplets  as they pass the light barrier. After dispensing, DropDetection produces color-coded and text-file-based results.


Key Benefits

1. Accuracy: Built-in droplet verification: I.DOT ‘s droplet detection  has the capability to detect when users run out of source liquid and can verify total dispense value.

2. Eliminate carryover and cross contamination: The technology enables droplets to be dispensed into the target  plate below the source plate.  I.DOT eliminates carryover and  cross-contamination.

3. Save time: I.DOT’s speed also saves you time per task from manual pipetting  and reduces repetitive work. 

4. Flexible: Dispense into any SBS target plate, including 96-, 384- and 1536-well plates, as well as customized labware.

5. Supports multiple liquid classes: Dispenses multiple liquid classes on-demand including aqueous solutions, various buffers, DMSO (up to 100%) and glycerol (up to 50%) and defines liquid class at the well level.

6.Save tips: I.DOT uses less consumables per task and does not depend on pipette tips which enable laboratories to budgets. With I.DOT’s low  dead volume, save 10 times  on reagents used. 

7. Low dead volume: Well reservoir design ensures dead volume < 1 µl for H20. 

8. Speed: Dispense 10 nl across a 96-well plate in 10 seconds and across a 384-well plate in 20 seconds.


Applications

High-throughput screening

Dispensing beads

Pooling libraries

Cell dispensing

Genomics and proteomics